Composite

Part:BBa_K3052033

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)


Light-fluoresce circuit (GFP) - DHJ

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The variables of this two-phases can be calculated by measuring the fluoresce intensity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4736
    Illegal PstI site found at 4817
    Illegal PstI site found at 5240
    Illegal PstI site found at 5306
    Illegal PstI site found at 5396
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6021
    Illegal NheI site found at 6044
    Illegal PstI site found at 4736
    Illegal PstI site found at 4817
    Illegal PstI site found at 5240
    Illegal PstI site found at 5306
    Illegal PstI site found at 5396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 7581
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4736
    Illegal PstI site found at 4817
    Illegal PstI site found at 5240
    Illegal PstI site found at 5306
    Illegal PstI site found at 5396
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4736
    Illegal PstI site found at 4817
    Illegal PstI site found at 5240
    Illegal PstI site found at 5306
    Illegal PstI site found at 5396
    Illegal NgoMIV site found at 4267
    Illegal NgoMIV site found at 7134
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None