Composite

Part:BBa_K3052031

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)


Light-fluoresce circuit (GFP) - LGF

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The variables of this two-phases can be calculated by measuring the fluoresce intensity of sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4502
    Illegal PstI site found at 5021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 5993
    Illegal NheI site found at 6016
    Illegal PstI site found at 4502
    Illegal PstI site found at 5021
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 4333
    Illegal BglII site found at 5295
    Illegal BglII site found at 5343
    Illegal BglII site found at 7553
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4502
    Illegal PstI site found at 5021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4502
    Illegal PstI site found at 5021
    Illegal NgoMIV site found at 4654
    Illegal NgoMIV site found at 7106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None