Composite

Part:BBa_K3052023

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


light controlling circuit - DHJ


This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. Besides, ho1 and pcyA are two essential parts for chromophore formation. The state of this two-phases switch can be altered by turning red light on and off, so that the selective production of linalool synthase and limonene synthase is approached.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6933
    Illegal NheI site found at 6956
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 8493
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
    Illegal NgoMIV site found at 5179
    Illegal NgoMIV site found at 8046
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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