Composite

Part:BBa_K3052022

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


light controlling circuit - RXY

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The state of this two-phases switch can be altered by turning red light on and off, so that the selective production of linalool synthase and limonene synthase is approached.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5531
    Illegal PstI site found at 6080
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6948
    Illegal NheI site found at 6971
    Illegal PstI site found at 5531
    Illegal PstI site found at 6080
    Illegal NotI site found at 5778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 6303
    Illegal BglII site found at 8508
    Illegal BamHI site found at 6085
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5531
    Illegal PstI site found at 6080
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5531
    Illegal PstI site found at 6080
    Illegal NgoMIV site found at 8061
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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