Composite

Part:BBa_K3040017

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


FadD

FadD is one of a regulatory enzyme in fatty acid biosynthesis in E. coli. , which is in charge of acetylating the fatty acid and produce a molecule, acetylated-fatty acid, that initiates promoter, pFadBA, to initiate the whole gene expression. We obtain the sequence of FadD directly by cell disruption of E. coli MG1655. After obtaining FadR, we further combine it with the promoter R0010 and a strong RBS B0030.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1395
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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