Part:BBa_K3040002
Lpp-OmpA-6X histag
Lpp-OmpA is an outer membrane protein that helps express the linked protein on the outer cell membrane. Lpp-OmpA is composed of two parts, lipoprotein, and the OmpA domain. Lipoprotein can target the outer cell membrane, which leads the whole protein binds to the membrane; and the OmpA domain is responsible for the expression of the linked protein. Modify and Improvement To express lipase on the outer cell membrane, we modified and improved the submitted Lpp-OmpA by iGEM15_NCTU-Formosa (BBa_K1694002 https://parts.igem.org/Part:BBa_K1694002). In our design, we replace the original NcoI restriction site with restriction sites AvrII and NheI which brings some benefits to the future application of Lpp-OmpA. First, we found that NcoI is a restriction site on the plasmid, pSB1C3, a commonly used plasmid in iGEM. Therefore, with this modification, Lpp-OmpA is able to apply to commonly used plasmid in iGEM. Furthermore, with the two designed restriction sites, we can link various proteins with Lpp-OmpA simply by adding AvrII and NheI restriction sites in front and behind the gene sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 619
- 1000COMPATIBLE WITH RFC[1000]
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