Part:BBa_K3030013

PP7 riboswitch + MS2 coat protein

This part is a combination of PP7 riboswitch and MS2 coat protein sequence to manipulate regulation process with another part automatically in the mammalian cells. We build up a three plasmids circuit which aimed to subsequently regulate expression level of gene(s) connected by 2A peptide with regulating protein (MS2/PP7).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Kennedy, A. B., Vowles, J. V., d'Espaux, L., & Smolke, C. D. (2014). 'Protein-responsive ribozyme switches in eukaryotic cells', Nucleic acids research, 42(19), 12306–12321. [Online]. Available from: https:// www.ncbi.nlm.nih.g ov/pubmed/18331713? dopt=Abstract

Lim,F & Peabody,D (2002) 'RNA recognition site of PP7 coat protein', Nucleic Acids Res, 2002 Oct 1; 30(19): 4138–4144. [Online]. Available from:https:// www.ncbi.nlm.nih .gov/pmc/articles/ PMC140551/