Composite
Part:BBa_K3028002
Designed by: Petr Zaytsev Group: iGEM19_Moscow (2019-10-15)
Composite cassette for sgRNA expression and binding assay
Two BBa_K1689000 parts carrying sgRNA spacer + fragment with targets for different CRISPR/Cas pairs
This part is a composite part of two BBa_K1689000 parts, which are sgRNA generators with already integrated spacers for two CRISPR/Cas complexes from Streptococcus pyogenes. The part is composed together with a fragment carrying targets for each of sgRNA. Targets are located on 21bp from each other and have a configuration of both PAM sites located out of targets.
This part is to be applied for the screening of split-reporter systems activities associated with CRISPR/Cas complexes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 235
Illegal XhoI site found at 800 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 425
Illegal BsaI site found at 990
Illegal BsaI.rc site found at 21
Illegal BsaI.rc site found at 586
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Categories
Parameters
None |