Composite

Part:BBa_K3028002

Designed by: Petr Zaytsev   Group: iGEM19_Moscow   (2019-10-15)


Composite cassette for sgRNA expression and binding assay

Two BBa_K1689000 parts carrying sgRNA spacer + fragment with targets for different CRISPR/Cas pairs

This part is a composite part of two BBa_K1689000 parts, which are sgRNA generators with already integrated spacers for two CRISPR/Cas complexes from Streptococcus pyogenes. The part is composed together with a fragment carrying targets for each of sgRNA. Targets are located on 21bp from each other and have a configuration of both PAM sites located out of targets.

This part is to be applied for the screening of split-reporter systems activities associated with CRISPR/Cas complexes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 235
    Illegal XhoI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 425
    Illegal BsaI site found at 990
    Illegal BsaI.rc site found at 21
    Illegal BsaI.rc site found at 586


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Parameters
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