Device

Part:BBa_K3024011

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-10-05)


Inducible cox characterization construct (Lytic module)

A composite, tri-cistronic part that Co-expresses TetR, Cox and GFP under the control of pBad promoter. Cox is a transcription factor known to activate downstream genes that impose the lytic cycle in P2 phage. TetR is an inhibitor acting on Tet-operetor regions (tetO).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1119
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 204
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1078
    Illegal BsaI.rc site found at 1785


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Categories
Parameters
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