Regulatory

Part:BBa_K3024010

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-28)


Double Promoter (Induced by arabinose, repressed by TetR)

The part consists of pBad promoter (BBa_K206000) and two repeats of the Tet Operator sequence (tetO). TetO is the responsive element that can be repressed by tetR and its sequence is coming from the part BBa_I739101. This is a double promoter that is at the same time inducible and repressible by two diferent molecules. Arabinose induces pBad whereas TetR inhibits downstream transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None