Device

Part:BBa_K3024004

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-28)


C-protein Characterization Construct (Lysogenic module)

Expresses C protein constitutively to keep P2 phage in the Lysogenic cycle upon infection. It can be inhibited by TetR, expressed by the TetR/cox expression module (part BBa_K3024011) which induces lysis. Contains RFP gene to be co-expressed with C protein as a live-cell marker. c protein is fused with a FLAG tag for immunological detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
    Illegal AgeI site found at 1109
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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