Device
Part:BBa_K302016
Designed by: Harsh Sheth, Alan Koh, Steven Woodhouse Group: iGEM10_Newcastle (2010-08-12)
Swarming
Bacillus subtilis 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:
- Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
- Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in swrA and thus the strain moves on the surface at a very slow pace.
This part restores the swarming ability of Bacillus subtilis 168.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 774
Illegal SapI.rc site found at 784
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
None |