Regulatory

Part:BBa_K302003

Designed by: Rachel May Boyd, Phil Hall and Jem Stach   Group: iGEM10_Newcastle   (2010-08-12)

P_hyperspankoid

This part is an IPTG-inducible promoter for B. subtilis adapted from pspacoid and hyper-spank.

It should have stronger LacI repression as it combines the improved lacI binding site (oid) from pspacoid with the repeat motif from hyper-spank. As a result, it should be less leaky.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None