Device

Part:BBa_K3011009

Designed by: Pinaki Chanda   Group: iGEM19_IISER_Kolkata   (2019-10-15)


Nitric Oxide sensor

SoxR (BBa_K554003) is a Nitric Oxide (NO) dependent transcription activator. This construct produces SoxR constitutively; in presence of NO, SoxR can activate the transcription of mRFP (BBa_E1010) which is under control of SoxS promoter (BBa_K554000). SoxR contains a Helix Turn Helix (HTH) motif by which it can bind to a particular DNA sequence (as a homodimer) and a [2Fe-2S] cluster. In presence of NO, [2Fe-2S] cluster reacts with NO to form dinitrosyl iron complex (Fig. 2.2), due to the formation of this complex SoxR can activate transcription of genes which is under control of SoxS promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1353
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None