Device

Part:BBa_K3011008

Designed by: Pinaki Chanda   Group: iGEM19_IISER_Kolkata   (2019-10-15)


Nitric Oxide sensor

NorR (BBa_K256004) is a Nitric Oxide (NO) dependent transcription activator. This construct produces NorR constitutively; in presence of NO, NorR can activate the transcription of mRFP (BBa_E1010) which is under control of pNO promoter (BBa_K256000). NorR contains a Helix Turn Helix (HTH) motif by which it can bind to a particular DNA sequence (as a homodimer). In presence of NO it can activate transcription of genes which is under control of pNO promoter. The regulatory domain of NorR contains a mononuclear non-haem iron centre (Fig. 2.1), which reversibly binds NO. Binding of NO stimulates the ATPase activity of NorR, enabling the activation of transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 703
    Illegal AgeI site found at 2397
    Illegal AgeI site found at 2509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 478


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Categories
Parameters
None