Coding

Part:BBa_K3011005

Designed by: Pinaki Chanda   Group: iGEM19_IISER_Kolkata   (2019-10-13)


iutA, involve in Aerobactin transportation in E. coli strains. It is ferric-aerobactin receptor

Aerobactin is an iron chelator (siderophore) produced by different strains of E. coli. It is a virulence factor enabling E. coli to sequester iron in iron-poor environments and helps bacteria in colonization inside the host. Aerobactin producing operon is a part of ColV plasmid of E. coli strains. Five genes are present on Aerobactin producing operon, four of them (iucA, iucB, iucC, iucD) are involved in Aerobactin synthesis and one (iutA) is responsible for transportation of Aerobactin.

Other parts involve in synthesis of Aerobactin:

iucA (BBa_K3011001) iucB (BBa_K3011002) iucC (BBa_K3011003) iucD (BBa_K3011004)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 849
    Illegal PstI site found at 667
    Illegal PstI site found at 892
    Illegal PstI site found at 1541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 849
    Illegal PstI site found at 667
    Illegal PstI site found at 892
    Illegal PstI site found at 1541
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 849
    Illegal BglII site found at 1713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 849
    Illegal PstI site found at 667
    Illegal PstI site found at 892
    Illegal PstI site found at 1541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 849
    Illegal PstI site found at 667
    Illegal PstI site found at 892
    Illegal PstI site found at 1541
    Illegal AgeI site found at 430
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None