Coding

Part:BBa_K3011001

Designed by: Pinaki Chanda   Group: iGEM19_IISER_Kolkata   (2019-10-13)


iucA, involve in Aerobactin biosynthesis in E. coli strains

Aerobactin is an iron chelator (siderophore) produced by different strains of E. coli. It is a virulence factor enabling E. coli to sequester iron in iron-poor environments and helps bacteria in colonization inside the host. Aerobactin producing operon is a part of ColV plasmid of E. coli strains. Five genes are present on Aerobactin producing operon, four of them (iucA, iucB, iucC, iucD) are involved in Aerobactin synthesis and one (iutA) is responsible for transportation of Aerobactin.

Other parts involve in synthesis of Aerobactin:

iucB (BBa_K3011002) iucC (BBa_K3011003) iucD (BBa_K3011004) iutA (BBa_K3011005)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1762
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1762
    Illegal NgoMIV site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None