DNA

Part:BBa_K300997

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-02)


AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix)

AvrII (BBa_B0048) and XbaI (BBa_M31991) restriction sites have a compatible overhang (CTAG).

The AvrII-XbaI mixed site is CCTAGA. When XbaI comes from RFC10 BioBrick Prefix, the DNA scar left after a ligation is CCTAGAG (see https://parts.igem.org/Help:BioBrick_Prefix_and_Suffix for details).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None