DNA

Part:BBa_K300996

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-02)


SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix)

SpeI (BBa_M31992) and AvrII (BBa_B0048) restriction sites have a compatible overhang (CTAG).

The SpeI-AvrII mixed site is ACTAGG. When SpeI comes from RFC10 BioBrick Suffix, the DNA scar left after a ligation is TACTAGG (see https://parts.igem.org/Help:BioBrick_Prefix_and_Suffix for details).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None