Intermediate

Part:BBa_K300982

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)


BBa_K300000 construction intermediate

This part was used to construct BBa_K300000 BioBrick integrative base vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1963
    Illegal XbaI site found at 1352
    Illegal XbaI site found at 2133
    Illegal SpeI site found at 1540
    Illegal PstI site found at 1980
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1963
    Illegal NheI site found at 1371
    Illegal NheI site found at 1787
    Illegal SpeI site found at 1540
    Illegal PstI site found at 1980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1963
    Illegal XbaI site found at 1352
    Illegal XbaI site found at 2133
    Illegal SpeI site found at 1540
    Illegal PstI site found at 1980
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1963
    Illegal XbaI site found at 1352
    Illegal XbaI site found at 2133
    Illegal SpeI site found at 1540
    Illegal PstI site found at 1980
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None