Protein_Domain

Part:BBa_K300095

Designed by: Giacomo Zambianchi, Alessandro Ranieri, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-22)

Phasin and intein separated by a flexible protein domain linker

Phasin (head domain) works as reported in BBa_K300002, while intein is described in BBa_K300004. This is a part composed of a phasin (head domain) and an intein separated by a 10aa flexible protein domain linker, which has the function to improve, enhance and ease the folding of fusion proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1078


[edit]
Categories
Parameters
None