Composite

Part:BBa_K300082

Designed by: Giacomo Zambianchi, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-17)

3OC6HSL inducible phasin generator

Phasin - head domain - works as reported in BBa_K300002. This composite part is intended to be built together to other fusion BioBrick parts (i.e. BBa_K300003 or reporter genes) in order to have a 3OC6HSL inducible synthesis of fusion proteins.

It was tested in BioBrick parts BBa_K300091 and BBa_K300092 with a combination of phasins (internal domain) (BBa_K300003)/linker (BBa_K105012)/intein (BBa_K300004) ligated downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


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Categories
Parameters
None