Composite

Part:BBa_K2996709

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-15)


LuxI downstream of activation unit containing lure

LuxI is put downstream of BBa_K2996704, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). We develop this to record off-target incidence. When the dcas9-sgRNA complex binds to target sequence, RpoA will recruit RNA polymerase, promoter J23117 will be activated. Then, by measuring the biological activity of the downstream reporter gene, we will know the effectiveness of this transcription activation device under different conditions.

Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage.

Figure 1. Gene circuit for transcription activation and information storage based on AHL


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None