Composite
Part:BBa_K2996708
Designed by: Yunqing Wang Group: iGEM19_SJTU-BioX-Shanghai (2019-10-15)
LuxI downstream of activation unit containing target
LuxI is put downstream of BBa_K2996704, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). When the dcas9-sgRNA complex binds to target sequence, RpoA will recruit RNA polymerase, promoter J23117 will be activated. Then, by measuring the biological activity of LuxI enzyme, we will know the effectiveness of this transcription activation device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 52
Illegal NheI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 729
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 39
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |