Composite

Part:BBa_K2996707

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-15)


mRFP downstream of activation unit containing lure

Florescent protein mRFP is put downstream of BBa_K2996705, as a reporter, where florescence intensity indicates the level of transcription activation. Compared to BBa_K2996706, we can get the probability that dcas9 binds to lure sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
    Illegal AgeI site found at 661
    Illegal AgeI site found at 773
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None