Composite

Part:BBa_K2996702

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-16)


tetracycline inducible transcription activator
To construct pActivator with or without sgRNA, RpoA-FL4-dcas9 is cloned downstream of the tetracycline inducible promotor.

Check BBa_K2996701 for more information.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None