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Part:BBa_K2996504

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


Binding sites for dCas9 and zinc finger

This part is used to test the proper spacer between the target of DBD binding site and target of NdCas9.The sequence is made up of the binding site of zincfinger and dCas9.The dCas9 in part BBa_K1689010 could bind to the target under the guidance of specific sgRNA.And the zinc finger protein in part BBa_K2996503 could bind to the DBD binding site.In this part,the spacer is 45 base pairs in length.We devised spacers of other length(5,15,25,35,45) by deleting base pairs on the basis of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 24
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 24
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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