Part:BBa_K299510

Expression Vector pT7+J6100

This part is licensed under
Creative BioCommons

This is expression vector containing J61100 under T7 promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.

Authors

Clonning:

• Ania Puławska
• Milena Bażlekowa

The lab work was supervised by:

• Michał Lower
• Ania Olchowik

Construct design

The device consists of T7 promoter followed by J61100 RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.

Expression strength

Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.

It's estimated to be 4,29% relative to BBa_K299509, the strongest ExpressIt! vector.

Summary for the whole ExpressIt! family is shown below:

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 33
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 33
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 33
  
• 1000
  COMPATIBLE WITH RFC[1000]