Part:BBa_K299327

J23100[promoter].K299107[TxbaIG].J61127[RBS] No b-brick scars between

J23100[promoter].K299107[xbaIG].J61127[RBS] = Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.

In this case the physical DNA mathes the registry sequence. After assembly part J61117 is in registry as AAAGAGTGGAAC, The functional DNA of this RBS (part J61127) is AAAGAGTGGAAC*T*, when suffix is added, the first T of the suffix gives 'AAAGAGTGGAACT' sequence.

This part is just to enable automatic in sillico assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.

The original Anderson RBSes and vector are described here.

https://parts.igem.org/wiki/index.php?title=Part:BBa_J61127

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 37
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 37
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 37
  
• 1000
  COMPATIBLE WITH RFC[1000]