Part:BBa_K299201
J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**
J23100[promoter].K299107[xbaIG].J61101[RBS] = Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
Also be careful part J61101 is in registry as AAAGACAGGACCC, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
AAAGACAGGACCC**a** (and then comes rest of the suffix: ctagt)
It means thatThe physical sequence is different than the record in registry for J61101, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.
This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
https://parts.igem.org/wiki/index.php?title=Part:BBa_J61101
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 45
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 45
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 45
- 1000COMPATIBLE WITH RFC[1000]
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