Part:BBa_K2991023

opti-pAraB reporter CFP

Description:

This allows you to study Opti-pAraB BBa_K2991003 (optimized promoter BBa_K2991000) with CFP.

What you can do with it:

In E.coli sugars are ranked depending on their capacity to increase growth rate. Promoters associated with each sugar operon will follow this hierarchy. Each part of the collection is made to exploit this Hierarchy (check our wiki,https://2019.igem.org/Team:Nantes).
You will learn more about it in this study ‘’G. Aidelberg, B. D. Towbin, D. Rothschild, E. Dekel, A. Bren, and U. Alon, “Hierarchy of non-glucose sugars in Escherichia coli,” BMC Syst. Biol., vol. 8, p. 133, 2014.’’

Compatibility:

Organism: We only tested this part in E.coli TOP10.
Backbone: We used the IDT plasmid with ampicillin resistance.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 216
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 265
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 216
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 216
  Illegal AgeI site found at 100
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 82