Part:BBa_K2984043

L1c-PsaD-MHETase-3xHA-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the MHETase enzyme, kindly provided by the iGEM TU Kaiserslautern 2019 team, a 3xHA-tag, and the Rbcs2 terminator. The part can be used to express the MHETase enzyme in C. reinhardtii. The 3xHA tag can be used to detect and purify the MHETase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1609
  Illegal PstI site found at 1933
  Illegal PstI site found at 2276
  Illegal PstI site found at 3086
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1609
  Illegal PstI site found at 1933
  Illegal PstI site found at 2276
  Illegal PstI site found at 3086
  Illegal NotI site found at 1944
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2854
  Illegal BamHI site found at 4
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1609
  Illegal PstI site found at 1933
  Illegal PstI site found at 2276
  Illegal PstI site found at 3086
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1609
  Illegal PstI site found at 1933
  Illegal PstI site found at 2276
  Illegal PstI site found at 3086
  Illegal NgoMIV site found at 1542
  Illegal NgoMIV site found at 2003
  Illegal NgoMIV site found at 2033
  Illegal NgoMIV site found at 2348
  Illegal NgoMIV site found at 2366
  Illegal NgoMIV site found at 2402
  Illegal NgoMIV site found at 3045
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Image of a successful transformation in E. coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii.