Coding

Part:BBa_K2984039

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsaD-PETase-3xHA-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the PETase enzyme, a HA tag, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1705
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1705
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC. reinhardtii
proteinPETase
tag3xHA