FadX of Punica granatum
This part is an Yarrowia lipolytica codon optimized version of the bifunctional fatty acid conjugase / desaturase (FadX) from Punica granatum (Uniprot Q84UB8).
Usage and Biology
The bifunctional fatty acid conjugase / desaturase FadX (EC: 22.214.171.124) catalyses the conversion of linoleic acid, C18:2 (9Z,12Z) into punicic acid, C18:3 (9Z, 11E, 13Z) (Figure 1).
The Punica granatum (pomegranate) FadX (Pg-FadX)  is a 45.828 kDa protein of 395 amino acids. It's activity leads to the accumulation of important quantities of punicic acid in the pomegranate seeds.
When placed them under the control of a constitutive promoter and expressed in the genome of the oleaginous yeast Yarrowia lipolytica, this part leads to the production of punicic acid, a non natural metabolite of Y. lipolytica. For further details, see the Main Page on the Registry of the Pg-FadX expression plasmid (BBa_K2983181) that is able to integrate, upon transformation, into the genome of a Y. lipolytica Po1d stain.
 Hornung E, Pernstich C, Feussner I. Formation of conjugated Delta11Delta13-double bonds by Delta12-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds. Eur J Biochem (2002) 269, 4852-4859.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC