Composite

Part:BBa_K2962006

Designed by: Sera Küçük   Group: iGEM19_Saint_Joseph   (2019-10-15)


pET26b with laccase enzyme from Trametes Versicolor.

PET 26b (BBa_K2962001), is an expression vector used for expression of Laccase gene in E. coli SHuffle organism and the Trametes versicolor laccase gene (Laccase (lcc1) BBa_K2962000) is an optimized sequence for expression in the E. coli organism. This composite part was used to transfer the laccase enzyme to shuffle.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 392
    Illegal BglII site found at 6147
    Illegal BglII site found at 6579
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
    Illegal XhoI site found at 6179
    Illegal XhoI site found at 6812
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 2012
    Illegal NgoMIV site found at 2172
    Illegal NgoMIV site found at 5219
    Illegal NgoMIV site found at 6665
    Illegal NgoMIV site found at 6818
    Illegal AgeI site found at 5546
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6101
    Illegal SapI site found at 3092
    Illegal SapI.rc site found at 5611


[edit]
Categories
Parameters
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