Plasmid_Backbone

Part:BBa_K2962001

Designed by: Handan KESIM   Group: iGEM19_Saint_Joseph   (2019-06-17)


PET26b

pET26b is a vector and laccase gene which codes laccase enzyme will be transferred to pET26b for production of the enzyme in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal BglII site found at 392
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 326
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 2012
    Illegal NgoMIV site found at 2172
    Illegal NgoMIV site found at 5219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 3092


[edit]
Categories
Parameters
None