Part:BBa_K2962000
lcc1 gene codes laccase enzyme.
Laccase enzyme is produced in Trametes versicolor which is known as white-rot fungus. Studies show that laccase can oxidize both toxic and nontoxic substrate. It can degrade dye and whiten the water. Today textile industries throw away wastewater after it has been processed with dye. By using laccase these wastewaters can be whitened and reused in the textile industry. Our project is producing this laccase enzyme in a bacteria called E.coli under laboratory conditions. Trametes versicolor laccase gene will be optimized in the first part of this study and then transferred into E.coli. After transformation of the laccase gene, it will be transferred to the expression vector to provide the expression of the enzyme.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 779
Illegal BglII site found at 1211
Illegal XhoI site found at 811
Illegal XhoI site found at 1444 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1450
Illegal AgeI site found at 178 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 733
Illegal SapI.rc site found at 243
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