Part:BBa_K2957095
NeonGreen-C5a
Contents: C5a, NeonGreen tag, with Strep resistance
What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
What does it do?: It codes for the chemokine, C5a, which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.
How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3730
Illegal SpeI site found at 2842
Illegal PstI site found at 223 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3730
Illegal SpeI site found at 2842
Illegal PstI site found at 223 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3730
Illegal BglII site found at 1691
Illegal BamHI site found at 239
Illegal XhoI site found at 1807 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3730
Illegal SpeI site found at 2842
Illegal PstI site found at 223 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3730
Illegal SpeI site found at 2842
Illegal PstI site found at 223 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3370
Illegal SapI.rc site found at 3343
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