Composite

Part:BBa_K2957036

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, synCD19

Contents: hEF1a, synCD19, SynthpA, with Amp resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and synCD19 reporter gene.

What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu

How to use it?: This part was used for transfection into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (a href=https://2019.igem.org/Team:MIT/Parts)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1231


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Categories
Parameters
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