Part:BBa_K2957013

CCL5 with NeonGreen tag (C-terminal)

Contents: CCL5 (RANTES), NeonGreen tag, with Carb resistance

What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a NeonGreen tag attached.

How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 442
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 442
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 458
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 442
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 442
  
• 1000
  COMPATIBLE WITH RFC[1000]