Composite

Part:BBa_K2957004

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-19)


CMV, IL-8 (CXCL-8)

Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1113
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1031
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1113
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1113
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1119
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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