Coding

Part:BBa_K2952005

Designed by: Charlotte von Koppenfels   Group: iGEM19_Edinburgh_UG   (2019-10-04)


HydEF

FeFe-Hydrogenase maturation factor. Highly conserved radical-SAM domains of both HydE and HydG and the GTPase domain of HydF are essential for achieving biosynthesis of active [FeFe] hydrogenases. The catalytic domain and a functionally complete set of Hyd maturation proteins are fundamental to achieving biosynthesis of catalytic [FeFe] hydrogenases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
    Illegal XhoI site found at 3016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1385
    Illegal NgoMIV site found at 1555
    Illegal NgoMIV site found at 2321
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None