Part:BBa_K2945002

Plasmid containing BBa_K2945000, and BBa_K325909 inserted into Psb1C3 backbone.

This plasmid contains two primary components: the first is BBa_K325909 which encodes the six necessary genes as well as their relavent component encoding for the production of bioluminescent blue light, the second is BBa_K2945000 which is a gene encoding for fatty acid photodecarboxylase. This part is intended to produce the fatty acid photodecarboxylase and the necessary blue light to catalytically activate it.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2033
  Illegal XbaI site found at 2048
  Illegal SpeI site found at 4073
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2033
  Illegal NheI site found at 5300
  Illegal NheI site found at 8266
  Illegal SpeI site found at 4073
  Illegal NotI site found at 2039
  Illegal NotI site found at 4080
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2033
  Illegal BglII site found at 7264
  Illegal BamHI site found at 5239
  Illegal XhoI site found at 1017
  Illegal XhoI site found at 1909
  Illegal XhoI site found at 8094
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 2033
  Illegal SpeI site found at 4073
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 2033
  Illegal XbaI site found at 2048
  Illegal SpeI site found at 4073
  Illegal NgoMIV site found at 2344
  Illegal NgoMIV site found at 2449
  Illegal NgoMIV site found at 3575
  Illegal AgeI site found at 5074
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2510
  Illegal BsaI site found at 3916
  Illegal BsaI site found at 9653
  Illegal BsaI.rc site found at 2713
  Illegal BsaI.rc site found at 6662
  Illegal SapI site found at 5056
  Illegal SapI.rc site found at 9978