Plasmid
NC64A

Part:BBa_K2945001

Designed by: Jacob Wolf   Group: iGEM19_UChicago   (2019-10-11)


Plasmid containing BBa_K2945000, inserted into Psb1C3 backbone.

This part contains the assembly of BBa_K2945000 into the standard iGEM backbone Psb1C3. This part encodes a fatty acid photodecarboxylase enzyme which converts fatty acids into alkanes in the presence of blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2033
    Illegal XbaI site found at 2048
    Illegal SpeI site found at 4073
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2033
    Illegal SpeI site found at 4073
    Illegal NotI site found at 2039
    Illegal NotI site found at 4080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2033
    Illegal XhoI site found at 1017
    Illegal XhoI site found at 1909
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2033
    Illegal SpeI site found at 4073
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2033
    Illegal XbaI site found at 2048
    Illegal SpeI site found at 4073
    Illegal NgoMIV site found at 2344
    Illegal NgoMIV site found at 2449
    Illegal NgoMIV site found at 3575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2510
    Illegal BsaI site found at 3916
    Illegal BsaI.rc site found at 2713


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Parameters
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