Coding

Part:BBa_K2944001

Designed by: Davindra Singh   Group: iGEM19_Concordia-Montreal   (2019-10-16)


Glucose oxidase optimized for Saccharomyces cerevisiae

Glucose oxidase from Aspergillus niger was codon optimized for transcription in Saccharomyces cerevisiae. Glucose oxidase catalyzes the oxidation of glucose to D-glucono-1,5-lactone and hydrogen peroxide. Original sequence was obtained from BBa_K2238000 and then codon optimized, but retains the His6-tag for purification.

This part is also flanked by assembly scars on both sides, between the promoter and the gene (5'-agatct-3') and as well between the gene and the terminator (5'-atcctaactcgag-3')

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 42
    Illegal BamHI site found at 352
    Illegal XhoI site found at 1916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 376
    Illegal BsaI.rc site found at 1594


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