Coding

Part:BBa_K2943006

Designed by: Erezyirmiya Yirmiya   Group: iGEM19_TAU_Israel   (2019-09-07)


prf15 (PAO1)/bacteriophage V10 tail fiber fusion

N terminal part of the tail fiber protein (prf15) from Pseudomonas aeruginosa PAO1 fused with the C terminal part of the V10 bacteriophage tail fiber protein. Allows Pyocin particles to targets e. coli EDL933 (O157:H7). The fused protein forms a tail that connects to the baseplate of the Pyocin on one side, and to the membrane of the rival bacteria on the other. This attachment is the first step in the process of attacking e. coli EDL933 (O157:H7). The fusion is of A.A 1 to 164 of the R2 type pyocin tail fiber and A.A 217 to 875 of the V10 tail spike.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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