Composite

Part:BBa_K2928013

Designed by: Lauren Hartz   Group: iGEM19_ASIJ_Tokyo   (2019-10-16)


Modified 0.19 Wheat Albumin (Gly to Glu) expression using TDH3, Secretion tag, RBS, and ADH3

This is a construct we used to express our modified 0.19 Wheat albumin, which acts as an alpha-amylase inhibitor. It can be transformed into yeast, and since there is a secretion tag, the 0.19 will be secreted into the solution or gel of the culture. Additionally, users should be aware that due to an amino acid substitution (the 4th amino acid, Gly, was changed to Glu), the attractions between amylase and the 0.19 were strengthened compared to the original 0.19 protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part
//chassis/eukaryote/yeast
Parameters
None