RBS
Part:BBa_K2927001
Designed by: KUO, PEI-CHING Group: iGEM19_CCU_Taiwan (2019-08-28)
Ribosome binding site
Ribosome entry site (RBS) recruits ribosome during the initiation of Cas12a protein translation.
Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Part Contributions
Group: iGEM22_Stony_Brook
Authors: Maulik Masaliya
Contribution Summary
This RBS is part of the Protein S gene circuit BBa_K4235030, in the vector BBa_K4235016. We successfully expressed protein S from bacterial expression system as highlighted on our team wiki.
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Categories
Parameters
None |