Composite

Part:BBa_K2926287

Designed by: Astrid T�bber   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)


Basic Insert to express Cas13a (Lbu) and a short guideRNA array in S. cerevisiae

adlsvkn. avd

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4795
    Illegal XbaI site found at 4589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4795
    Illegal NheI site found at 4614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4795
    Illegal BglII site found at 838
    Illegal BglII site found at 1774
    Illegal BglII site found at 2038
    Illegal BglII site found at 2542
    Illegal BglII site found at 3028
    Illegal BglII site found at 3136
    Illegal BglII site found at 3466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4795
    Illegal XbaI site found at 4589
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4795
    Illegal XbaI site found at 4589
    Illegal AgeI site found at 105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3147


[edit]
Categories
Parameters
None