Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Fusion protein of Opy2 from yeast, mCherry and pVIII from the bacteriophage M13
The extracellular domain of S. cerevisiaes membrane protein Opy2 was N-terminally fused to mCherry to enable protein visualization. Additionally, the major coat protein pVIII of the bacteriophage M13 was fused to the C-terminus of mCherry.
Usage and Biology
This part was used during Troygenics Assembly to visualize the produced M13 bacteriophage-derived Troygenics via fluorescence measurement (Fig. 1).
We compared the emission spectrum of our Troygenics with the emission spectrum of mCherry, because the fluorescence marker cloned on the Troygenics is mCherry, too. We have seen a difference in the emission peak of about 20 nm. This is probably because the mCherry on our Troygenics is fused to the whole Troygenic. Because of this, there might be a different folding resulting in a shift of the emission spectrum.